Topic outline

  • Course Information

    Collapse all Expand all

    Course informationFaculty: Sciences

    Department: Common Trunk Nature and Life Sciences

    Target audience: 1st year TC license

    Course title: Cellular Biology Laboratory (Cell biology Lab)

    Credit: 08

    Coefficient: 04

    Duration: 15 weeks (90h)

    Hourly volume: 3h00 Classes – 3h00 Practical work

    Evaluation method: 

       - Continuous assessment: 40%

       - Final exam: 60%.

    Hourly:

       - Sunday: 8:00 a.m. - 11:00 a.m. Amphi: D01

       - Monday / Tuesday: 8:00 a.m. - 11:00 a.m. I-20 Lab



  • Contact information

      - Cellular biology laboratory Teacher: Dr. Zakaria HEDJOULI

      - Contact : zakaria.hedjouli@univ-msila.dz

      - Availability:
        - At the teachers' office (D09): Sunday, Monday, Tuesday from 8:30 a.m. to 9:30 a.m.
        - Answer on the forum: any question relating to the course must be posted on the dedicated forum so that you can all benefit from my answer, I undertake to answer the questions posted within 48 hours.
      - By email: 
        - I undertake to respond by email within 48 hours following receipt of the message, except in the event of unforeseen circumstances, I draw your attention to the fact that the preferred communication channel is the forum. 



    "Note : The email is reserved for "emergencies” (in the event of a problem accessing the platform) and it must be used with discernment."


  • Content


    • Objectives
    • Introduction

    I)- Laboratory safety rules

         I.1. Personal Protective Equipment (PPE)

         I. 2. General Safety Practices

         I. 3. Additional Cautions

    II) - 1st Lab work - Compound Light Microscope

         II. 1. Objectives of 1st lab work

         II. 2. Introduction

         II. 3. The different parts of the microscope

         II. 3.1. The functions of the different parts of the microscope

         II. 4. Principle of a light microscope (optical microscope)

         II. 5. Instructions for using the microscope

         II. 6. Activity 1

         II. 7. Activity 2

         II. 7.1. Materials

         II. 7.2. Applications

         II. 7.3. Observation under the microscope

         II. 8. Final Analysis

    III) - 2nd Lab work - Microscopic study of Animal cells (Blood smear)

         III. 1. Objectives of 2nd lab work

         III. 2. Introduction

         III. 3. Blood Components

         III. 3.1. What is Blood?

         III. 3.2. Components of Blood

         III. 3.3. Types of White Blood Cells

         III. 4. Preparing Blood Smear for observation

         III. 4.1. Materials

         III. 4.2. Blood Collection

         III. 4.3. Staining the Blood Smear

         III. 5. Microscope Observation

         III. 6. Final Analysis

    IV) - Exit test

  • Course Mind map

    Mind map of Cellular biology laboratory

  • Objectives

    Cell biology lab work aims to provide students with hands-on experience and practical knowledge in various areas related to the structure, function, and behavior of cells. These labs can be designed to achieve various objectives, including:

       1. Develop practical skills in cell biology techniques: Students will learn and practice essential techniques used in cell biology research and applications.

       2. Distinguish the cell biology concepts: Through practical experience, students will solidify their understanding of theoretical concepts learned in lectures.

       3. Construct critical thinking and problem-solving skills: encourages students to formulate hypotheses, analyze and interpret data, troubleshoot and adapt procedures.

       4. Create scientific communication and collaboration: often involves working in teams, promoting: Collaborating between students and scientific communication.


  • Introduction


    Cell biology (also cellular biology) is a branch of biology that studies the structure, function, and behavior of cells. All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and functioning of organisms.

    Cell biology is the study of the structural and functional units of cells. Cell biology encompasses both prokaryotic and eukaryotic cells and has many subtopics which may include the study of cell metabolism, cell communication, cell cycle, biochemistry, and cell composition.

    The study of cells is performed using several microscopy techniques, cell culture, and cell fractionation. These have allowed for and are currently being used for discoveries and research pertaining to how cells function, ultimately giving insight into understanding larger organisms.

    Knowing the components of cells and how cells work is fundamental to all biological sciences while also being essential for research in biomedical fields such as cancer, and other diseases. Research in cell biology is interconnected to other fields such as genetics, molecular genetics, molecular biology, medical microbiology, and immunology.


  • Prerequisites

    The student should typically have a strong foundation in biology, chemistry, physics, and sometimes even math to study this module.

    Biology: This is the foundation for understanding cell biology. A general biology course will introduce you to the basic concepts of life, including cell structure and function, genetics, and evolution.

    Organic Chemistry: Organic chemistry is the study of carbon based molecules, which are the building blocks of life. While not always required, understanding organic chemistry can be helpful for understanding the structure and function of biological molecules, such as proteins, carbohydrates, and lipids.

    Physics: provides a foundation for understanding the physical principles that govern cellular processes, such as diffusion, osmosis, and thermodynamics. You'll likely need to cover topics like mechanics, electricity, and magnetism.

    Calculus: Although not always required, calculus can be helpful for understanding some of the quantitative aspects of cell biology, such as modeling cellular processes.



  • Prerequisite Test

    • resource icon
      File
      Click here to start the Quiz File
      View



      Notice :

      All Students are invited to test there knowledge before starting this course, to make sure they have the prerequisite needed to study this module.


    • googlemeet icon
      Google Meet™ for Moodle
      Meeting with your professor Google Meet™ for Moodle

      - The first meeting with your professor will help you for clarification and guidance 

      - If you have any questions about the syllabus, course expectations, or challenging concepts 

      - The first meeting is a perfect opportunity to seek clarification directly from the source. This can help you get off on the right foot and avoid confusion later.

  • Laboratory safety rules

  • How to write a Lab report



  • Lab class 01 : Compound Light Microscope

                                                  

  • Bibliographic references

    1.   Alberts, B., Hopkin, K., Johnson, A. D., Morgan, D., Raff M. C., Roberts, K., Walter, P. (2019). Essential Cell Biology. United Kingdom: W.W. Norton. ISBN: 9780393680393

    2.   Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2002). Molecular biology of the cell, Bruce Alberts...[et al.]. Garland Science. New York. US. ISBN-10: 0-8153-4072-9

    3.   Kierszenbaum, A.L., Tres, L. (2011). Histology and Cell Biology: An Introduction to Pathology E-Book. United Kingdom: Elsevier Health Sciences. ISBN: 9780323683784.

    4.   Thomas Dean Pollard & William C. Earnshaw, (2004). Biologie cellulaire. Ed. Elsevier Masson, Paris, 853p.

    5.   Marc Maillet, (2006). Biologie cellulaire. Ed. Elsevier Masson, Paris, 618p.


  • Your Thoughts